Изучение влияния гуморальных факторов гомеостатической пролиферации на Т-регуляторные клетки in vitro

Abstract

Role of T-regulatory cells (Treg) in pathogenesis of different autoimmune diseases is widely known. A lot of studies have been dedicated to contribution of these cells in development of rheumatoid arthritis. It was shown that different abnormalities of these cells such as quantitative or qualitative may lead to beginning and progression of autoimmune pathology. At the same time some authors note the importance of homeostatic proliferation (HP) in beginning of autoimmune diseases. HP is normal physiological process caused by lymphopenia to support constant level of T cells. However, HP can make some negative contribution. This process accompanied by decrease of T-cell receptor (TCR) repertoire and selection of T-cell clones with relatively high affinity to selfantigens. In common this may increase the risk of development of autoimmune pathology. However, impact of homeostatic proliferation on Treg cells is not completely understood. In this study we performed the in vitro assay of influence of the main humoral factors of HP - IL7 and IL15 cytokines on Treg cells. We revealed these factors, especially IL7, can support surviving and stability Treg cells phenotype (by FoxP3 expression) and can increase the expression of functional molecules of Treg cells such as PD-L1 in experiment which was performed on cells obtained from healthy donors blood. So these results reflect the importance of homeostatic proliferation and its humoral factors in not only supporting of conventional T-cells but in influence on T-regulatory cells. It can play significant role in the development of autoimmune diseases. So we are going to continue our investigations with blood from rheumatoid arthritis patients.