Please use this identifier to cite or link to this item: http://earchive.tpu.ru/handle/11683/57327
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dc.contributor.authorKhristunova, Ekaterinaen
dc.contributor.authorKorotkova, Elena Ivanovnaen
dc.contributor.authorKratochvil, Bohumilen
dc.contributor.authorBarek, Jirien
dc.contributor.authorDorozhko, Elena Vladimirovnaen
dc.contributor.authorVyskocil, Vlastimilen
dc.contributor.authorPlotnikov, Evgeny Vladimirovichen
dc.contributor.authorVoronova, Olesya Aleksandrovnaen
dc.contributor.authorSidelnikov, Vladimir Sergeevichen
dc.date.accessioned2020-01-17T05:43:24Z-
dc.date.available2020-01-17T05:43:24Z-
dc.date.issued2019-
dc.identifier.citationPreparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis / E. Khristunova [et al.] // Sensors. — 2019. — Vol. 19, iss. 9. — [2103, 10 p.].en
dc.identifier.urihttp://earchive.tpu.ru/handle/11683/57327-
dc.description.abstractA new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL?1 to 1600 IU·mL?1, with a detection limit of 50 IU·mL?1. The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.en
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherТомский политехнический университетru
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.sourceSensorsru
dc.subjectbioconjugatesru
dc.subjectsilver nanoparticlesru
dc.subjectantibodiesru
dc.subjecttick-borne encephalitis virusru
dc.subjectgold–carbon composite electroderu
dc.subjectelectrochemical immunosensorru
dc.subjectбиоконъюгатыru
dc.subjectнаночастицыru
dc.subjectантителаru
dc.subjectвирусыru
dc.titlePreparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitisen
dc.typeArticleen
dc.typeinfo:eu-repo/semantics/publishedVersionen
dc.typeinfo:eu-repo/semantics/articleen
dcterms.audienceResearchesen
local.description.firstpage2103-
local.filepathhttps://doi.org/10.3390/s19092103-
local.identifier.bibrecRU\TPU\network\30194-
local.identifier.perskeyRU\TPU\pers\39741-
local.identifier.perskeyRU\TPU\pers\31278-
local.identifier.perskeyRU\TPU\pers\32174-
local.identifier.perskeyRU\TPU\pers\32469-
local.identifier.perskeyRU\TPU\pers\32169-
local.issue9-
local.localtypeСтатьяru
local.volume19-
dc.identifier.doi10.3390/s19092103-
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