Получение антимикробного пептида UBI[18-35] с использованием рекомбинантных технологий

Abstract

Currently, short peptides are widely used in diagnostics. These molecules not only provide the high-avidity binding with target cells but also have smaller sizes in comparison with the proteins. Peptides are nearly invisible to the immune system and are expected to cause minimal or no side effects. Compared with isolation from natural sources and chemical synthesis, recombinant approach offers the most cost-effective technology for large-scale peptide manufacture. The aim of this work was to obtain the recombinant E.coli strain, transformed by vector construction, encoding expression of UBI18-35 peptide. The UBI18-35 antimicrobial peptide labeled radionuclide marker can be applied for detection of pathological processes arising from microbial infection.