Оптимизация метода получения моноцитов для культивирования и программирования IN VITRO

Abstract

Monocytes are considered to be precursors of the mononuclear phagocytes system. Due to the wide variety of macrophages functions nowadays investigation of monocytes and macrophages functions, properties and reactions is one of the most actual. The aim of the study was to choose and optimize a method of monocyte isolation from healthy donors. The result of our research as supposed was to receive the maximum number of cells with a purity that allows to carry out functional tests in vitro and to evaluate the expression of monocyte genes of interest. As a result, the protocol of monocyte isolation from human peripheral blood with CD14- positive selection with magnetic separation (MACS) was held. Optimal concentrations of cytokines for monocyte stimulation and for modelling monocyte properties in vitro in cell culture were found for further researches.