Ген флавоноид 3', 5'-гидроксилазы ячменя как мишень для редактирования

Abstract

Secondary metabolites anthocyanins are synthesized by many higher plants including an important agricultural crop barley (Hordeum vulgare L.). The study of genes involved in the synthesis of anthocyanins is actual because of health benefits of these compounds. One of the least studied structural genes in this metabolic pathway are genes of cytochrome P450 family. The aim of this study was to identify, compare, and analyze duplicated paralogous copies of cytochrome P450-encoding flavonoid 3', 5'-hydroxylase genes in a barley genome. We identified four F3'5'H gene copies in the barley genome using a database for not annotated sequences using BLAST. The available sequences have cytochrome P450 domain. One of the copies carries a frame-shift mutation damaging the functional P450 domain, which could lead to changes in gene function. The transcription activity of the detected genes in various parts of plant varied among the copies. It is assumed that the maintenance of these duplicated genes is probably related to their specialization - all copies of F3'5'H can participate in the synthesis of anthocyanin compounds in different parts of the plant.