Изменение экспрессии рецепторов опухолеассоциированных макрофагов под воздействием цисплатина

Abstract

In the present study, the analysis of the effect of cisplatin on the expression of the receptors, responsible for the internalization of components of tumor microenvironment, was carried out on the model tumor-associated macrophages (TAM). We performed PCR quantitative analysis of the expression of CD206, CD163, LYVE1, stabilin-1 - the main scavenger receptors expressed on the TAM, and the analysis of the surface expression by confocal microscopy allowed to establish the differences in the effect of cisplatin on model TAM of breast adenocarcinoma and human colorectal carcinoma. It was found that cisplatin has suppressing effect on the scavenging potential of macrophages, except the increasing of the potential for endocytosis of hyaluronic acid in model macrophages of breast tumor, but not of colorectal cancer.