Исследование лимфедемы методом многофотонной микроскопии высокого разрешения

Abstract

In this paper results of iv-vivo measurements for healthy volunteers and people, with verified diagnosis of lymphedema, obtained with two-photon tomography with FLIM mode on MPTflex equipment (JenLab GmbH) are presented. In the study, eight healthy people and eight people with lymphedema disease participated. The papillary layer of the skin was analyzed at a depth of about 100 µm. In the course of the study, a small redistribution of autofluorescence lifetimes was observed for healthy volunteers and people with lymphedema disease. However, the main component composition in similar parts of the papillary dermis don't not change significantly. This allows to make assumption that as a result of the development of the disease, structural changes occur that do not significantly affect the component composition of the tissue.