Оценка противоопухолевой эффективности различных видов ИПМИ в сочетанном действии с доксорубицином in vitro

Abstract

It is known that the action of high-amplitude electric pulses can significantly increase the permeability of cell membranes due to the formation of pores in them (electroporation). The aim of this research was to study the possibility of forming electropores in membranes of tumor cells by means of a non-invasive method using nanosecond microwave pulses for effective delivery of chemotherapeutic agents to the cell. The HeLa cell line was used as a model object. Doxorubicin was used as a chemotherpeutic drug in various concentrations: 0.5, 1.0 and 2.5 [mu]M. Radiation was carried out on two sources of pulsed-periodic microwave radiation, characterized by different parameters. It was found that, 24 hours after exposure at pulse repetition rates of 13 and 16 Hz in combination with doxorubicin, there was an increase in inhibition of tumor cell proliferation by 30 and 40 %, respectively.