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Title: Циркулирующие ДНК-маркеры при мониторинге рака лёгкого: анализ статуса метилирования ретроэлементов LINE-1
Other Titles: Circulating DNA markers in monitoring lung cancer: analysis of LINE-1 retroelement methylation status
Authors: Умарова, Л. А.
Пономарева, А. А.
metadata.dc.contributor.advisor: Чердынцева, Н. В.
Keywords: ДНК; маркеры; мониторинг; метилирование; рак легких; онкологические заболевания; сравнительный анализ; кровь; противоопухолевая терапия
Issue Date: 2018
Publisher: Издательский Дом Томского государственного университета
Citation: Умарова Л. А. Циркулирующие ДНК-маркеры при мониторинге рака лёгкого: анализ статуса метилирования ретроэлементов LINE-1 / Л. А. Умарова, А. А. Пономарева ; науч. рук. Н. В. Чердынцева // Перспективы развития фундаментальных наук : сборник научных трудов XV Международной конференции студентов, аспирантов и молодых ученых, г. Томск, 24-27 апреля 2018 г. : в 7 т. — Томск : Издательский Дом Томского государственного университета, 2018. — Т. 4 : Биология и фундаментальная медицина. — [С. 155-157].
Abstract: It is known that the violation of DNA methylation processes is one of the earliest and most common events in malignant tumors. The purpose of this study was to perform a comparative analysis of the methylation level of LINE-1 retroelements in blood cirDNA in patients with lung cancer before treatment and at the stages of dynamic observation after antitumor therapy. The study included 16 patients with non-small cell lung cancer (NSCLC). The material for the study was venous blood, which was taken before the treatment (10-15 days before the start of treatment), 15-30 days after the end of the last course of non-adjuvant chemotherapy and in the postoperative period for 10-15 days. Analysis of the methylation level of LINE-1 element was performed using a quantitative methyl specific PCR .A linear increase in the methylation index of the LINE-1 fragment in response to antitumor therapy was revealed: after chemotherapy, a statistically significant increase in the methylation index was approximately 2-fold (p = 0.04, paired t-test), 1.4 times after operation indicators after chemotherapy (p = 0.134, paired t-test). As a result, after combined treatment, the LINE-1 methylation index in cc-cirDNA increased 3-fold compared to the level before treatment (p = 0.03, paired t-test). The obtained results indicate the prospectivity of the study on extended samples of lung cancer patients with the significance of the LINE-1 methylation index in blood cirDNA for predicting tumor response to treatment, evaluating the effectiveness of therapy and early detection of relapses.
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