Новые электрохимические сенсоры для вольтамперометрического определения интерферонов

Abstract

In this paper, we consider the possibility of using the voltammetric method to determine alpha- and gamma-interferon using an analytical complex that includes a polarizable working electrode, an auxiliary electrode, and a non-polarizable reference electrode. Traditional carbon electrodes used to determine organic compounds do not have sufficient sensitivity to analyze interferons in biological environments, which necessitated modifying the surface of the working glass carbon electrode. The auxiliary and reference electrodes were made on the basis of silver chloride. To increase the sensitivity of interferon determination, a carbon electrode with a modified surface is proposed. Gold and aryldiazonium salts with a carboxyl group were used as modifiers. The use of such an organo-modified electrode provided clear signals of alpha- and gamma-interferons in a background phosphate buffer solution with pH 9.18. The analysis results showed a linear dependence of the peak height on the concentration of interferons. It was also found that the maximum peak height is observed at the accumulation potential of -0.6 V. Optimization of the analysis conditions, such as the electrode material, accumulation potential, accumulation time and composition of the Electrolyte backgrounds, was successfully carried out. The use of gold for electrode modification is presented as one of the ways to increase the sensitivity of the method