Разработка клеточ ной тест-системы на основе зародышевой формы Вич-специфичного широко нейтрализующего антитела 10E8

Abstract

Identification of antigens driving affinity maturation of broadly neutralizing antibodies is one of the most challenging endeavors in the HIV vaccine development field. In order to isolate such candidate immunogens and to analyze whether they may activate B-cells expressing BCRs based on immature/germline forms of broadly neutralizing antibodies, a robust test system that faithfully recapitulates human B-cell biology would be indispensable. Thus, developing a human В-cell sensor line with surface expression of a germline variant of broadly neutralizing antibody 10E8 may represent one of the possible solutions.