Определение цитотоксического эффекта микроорганизмов, выделенных из пациентов с болезнью Паркинсона, на клеточной линии U251

Abstract

In the present study, the cytotoxicity of microbial communities and pure cultures of bacteria obtained by the method of cultivation from patients with Parkinson's disease was analyzed in a human U251 glioma cell line. Co-cultivation of the U251 cell line was carried out for four and eight hours with accumulative bacterial cultures and pure cultures of Clostridium symbiosum, obtained from the microbiota of patients with Parkinson's disease, and Escherichia coli, a collection strain XL-blue. The cytotoxicity of microorganisms belonging to the genus of Clostridium, Ruminococcus and Eggerthella, particularly to the Ruminococcus gnavus, Clostridium celatum, Eggerthella lenta and Clostridium symbiosum, was shown during co-cultivation with the cell line. A cytotoxic effect was also shown through the co-cultivated with the pure Clostridium symbiosum culture.