Разработка метода получения и очистки CEdG для молекулярных и биологических исследований

Abstract

Methylglyoxal (MG) is a high reactive compound and it is one of the damaging environmental factors. Methylglyoxal presents in micromolar quantities in many food products and in most biological fluids of different organisms, it is known that increasing of MG level has a mutagenic effect in vivo [1]. MG is capable of reacting with nucleophilic groups in proteins, lipids and DNA, forming covalent compounds also known as advanced glycation end products. Protein glycation end products have been characterized well and it was shown that these adducts play a role in various pathological processes, such as diabetes mellitus [2], cancer, degenerative conditions associated with aging [3] Alzheimer's disease. DNA glycation end products can be used as potential markers of different pathological processes. N2-carboxyethyl-2'-deoxyguanosine is one of the compounds which formed during the reaction of methylglyoxal or glucose with double-stranded DNA in vitro [5].The aim of the research was to synthesize of N2-carboxyethyl -2'-deoxyguanosine as a standard substance for molecular studies of the DNA glycation in the socially significant diseases pathogenesis. We propose a new method for purifying N2-carboxyethyl-2'-deoxyguanosine from the reaction mixture by reversed-phase HPLC.