Фенотипические особенности полиморфизма гена интерлейкина-6 при формировании аддиктивной патологии

Abstract

Search for biomarkers of addictive disorders including molecular-genetic and immunoregulatory characteristics is of relevance. 114 persons with mental and behavioural disorders caused by use of psychoactive substances and 97 conditionally healthy persons have been examined. The assessment of interleukin-6 gene polymorphism rs1800797 has been carried out with use of real-time PCR detection system. Level of extracellular mitogen-induced production of interleukin-6 has been measured on the Luminex 200 multiplex analyzer (USA). Intracellular contents of interleukin-6 have been estimated in mononuclears of stimulated LPS culture of whole blood on the FACS Calibur flow cytofluorimeter (BD, USA). Narcological patients are characterized by higher mitogen-induced production of interleukin-6. Persons with GG genotype of interleukin-6 gene polymorphism rs1800797 have demonstrated significant increase of production of interleukin-6. The GG genotype of interleukin-6 gene polymorphism rs1800797 makes the maximal contribution to formation of mental and behavioural disorders caused by use of psychoactive substances and can be a marker of addictive pathology.