Please use this identifier to cite or link to this item: http://earchive.tpu.ru/handle/11683/57555
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dc.contributor.authorKhlusov, Igor Albertovichen
dc.contributor.authorDekhtyar, Yurien
dc.contributor.authorSharkeev, Yury Petrovichen
dc.contributor.authorPichugin, Vladimir Fyodorovichen
dc.contributor.authorKhlusova, Marina Yurjevnaen
dc.contributor.authorPolyaka, Nataliyaen
dc.contributor.authorTyulkin, Fyodoren
dc.contributor.authorVendinya, Viktorijaen
dc.contributor.authorLegostaeva, Elena Viktorovnaen
dc.contributor.authorLitvinova, Larisa Sergeevnaen
dc.contributor.authorShupletsova, Valeria Vladimirovnaen
dc.contributor.authorKhaziakhmatova, Olga Gennadjevnaen
dc.contributor.authorYurova, Kristina Alekseevnaen
dc.contributor.authorYurova, Kristina Alekseevnaen
dc.contributor.authorProsolov, Konstantin Aleksandrovichen
dc.date.accessioned2020-01-23T09:34:51Z-
dc.date.available2020-01-23T09:34:51Z-
dc.date.issued2018-
dc.identifier.citationNanoscale Electrical Potential and Roughness of a Calcium Phosphate Surface Promotes the Osteogenic Phenotype of Stromal Cells / I. A. Khlusov [et al.] // Materials. — 2018. — Vol. 11, iss. 6. — [978, 25 p.].ru
dc.identifier.urihttp://earchive.tpu.ru/handle/11683/57555-
dc.description.abstractMesenchymal stem cells (MSCs) and osteoblasts respond to the surface electrical charge and topography of biomaterials. This work focuses on the connection between the roughness of calcium phosphate (CP) surfaces and their electrical potential (EP) at the micro- and nanoscales and the possible role of these parameters in jointly affecting human MSC osteogenic differentiation and maturation in vitro. A microarc CP coating was deposited on titanium substrates and characterized at the micro- and nanoscale. Human adult adipose-derived MSCs (hAMSCs) or prenatal stromal cells from the human lung (HLPSCs) were cultured on the CP surface to estimate MSC behavior. The roughness, nonuniform charge polarity, and EP of CP microarc coatings on a titanium substrate were shown to affect the osteogenic differentiation and maturation of hAMSCs and HLPSCs in vitro. The surface EP induced by the negative charge increased with increasing surface roughness at the microscale. The surface relief at the nanoscale had an impact on the sign of the EP. Negative electrical charges were mainly located within the micro- and nanosockets of the coating surface, whereas positive charges were detected predominantly at the nanorelief peaks. HLPSCs located in the sockets of the CP surface expressed the osteoblastic markers osteocalcin and alkaline phosphatase. The CP multilevel topography induced charge polarity and an EP and overall promoted the osteoblast phenotype of HLPSCs. The negative sign of the EP and its magnitude at the micro- and nanosockets might be sensitive factors that can trigger osteoblastic differentiation and maturation of human stromal cells.en
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherТомский политехнический университетru
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.sourceMaterialsru
dc.subjectsurface roughnessru
dc.subjectsurface electrical potentialru
dc.subjectmicro- and nanoscaleru
dc.subjecthuman mesenchymal cellsru
dc.subjectosteocalcinru
dc.subjectalkaline phosphataseru
dc.subjectin vitroru
dc.subjectшероховатостиru
dc.subjectэлектрические потенциалыru
dc.subjectмикроразмерные устройстваru
dc.subjectнаноразмерностьru
dc.titleNanoscale Electrical Potential and Roughness of a Calcium Phosphate Surface Promotes the Osteogenic Phenotype of Stromal Cellsen
dc.typeArticleen
dc.typeinfo:eu-repo/semantics/publishedVersionen
dc.typeinfo:eu-repo/semantics/articleen
dcterms.audienceResearchesen
local.description.firstpage978-
local.filepathhttps://doi.org/10.3390/ma11060978-
local.identifier.bibrecRU\TPU\network\29257-
local.identifier.perskeyRU\TPU\pers\34907-
local.identifier.perskeyRU\TPU\pers\32228-
local.identifier.perskeyRU\TPU\pers\30933-
local.issue6-
local.localtypeСтатьяru
local.volume11-
dc.identifier.doi10.3390/ma11060978-
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