Повышение количества астроцитов в модели тотальной ишемии головного мозга у крыс

Abstract

Astrocytes are the most abundant cell class in the CNS. Astrocytic therapies have huge potential for neuronal repair after stroke. The most number of brain stroke studies address to clear damage of neurons. Modern studies turn to usage of morphological and functional changes in astroglial cells after stroke in regenerative medicine. Our study is focused on the changes of numbers of astrocytes in hippocampus (where new glia divide) after brain ischemia. Ischemia was modeled by occlusion of tr. brachiocephalicus, a. subclavia sin., a. carotis communis sin. Astrocytes were determined using immunohistochemical labeling with anti GFAP antibody. We found out that number of astrocytes increased in 10th and 30th days after stroke in CA1, СА2 fields, granular layer of dentate gyrus (GrDG) and hilus. Morphology of astrocytes became reactive in these regions. Our results reveal substantial long-term inflammation in hippocampus region after global ischemia in rats.