Реакция стволовых и прогениторных клеток на эмфизему легких

Abstract

We studied inflammation, proteins of extracellular matrix, stem and progenitor cells from female C57Bl/ 6 mice on the model of elastase-induced emphysema. It was shown that diffuse emphysema reduces the amount of endothelial (CD45-CD31+ CD34+) and epithelial cells (CD45-CD117+CD49f+), the area of the connective tissue, causes a disturbance of microcirculation. The pulmonary emphysema is accompanied by infiltration of macrophages and lymphocytes in alveolar septum. Simultaneously with inflammation in the lung we observed an increase of the count of multipotentmesenchymal stromal cells (CD45-CD90+CD73+CD106+CD44+) and overexpression of extracellular matrix proteins (hydroxyproline, connective tissue growth factor, collagen, fibronectin), Clara cell protein and Clara cells (CD45-CD34-CD31-Sca1+). On the contrary elastase reduces the count of angiogenesis progenitor (CD45-CD117+ Flk1+).