Влияние инсулин оподобного фактора роста 1 на патологические процессы в купризоновой модели рассеянного склероза

Abstract

The study aimed to evaluate the effect of insulin-like growth factor 1 (IGF-1) on the cuprizone-induced murine model of demyelination and accompanying astrogliosis. Demyelination was induced in 14 adult male mice by 0.3% cuprizone in drinking water. Five animals from the cuprizone-teated group received subcutaneous injections of IGF-1. Seven animals were used as a control group. The extent of demyelination was evaluated as a decrease of the size of the corpus callosum on T2-weighted images that were received using 11.7T animal MRI scanner. Brain sections were immunohistochemically stained for glial fibrillary acidic protein (GFAP), a marker of astrocytes. It was revealed the cuprizone caused extensive demyelination and astroglyosis. IGF-1 treatment restored the size of corpus callosum and the number of astrocytes in corpus callosum and anterior commissure to the control level.