Генетически модифицированная клеточная линия с гиперэкспрессией гена MYC

Abstract

We have previously shown that cell lines carrying amplifications of two or more stemness genes are capable of dedifferentiation with the formation of mammospheres. The BT549 cell line has amplification of only one stemness gene, KLF5, and is incapable of dedifferentiation. In the present study, a monoclonal breast cancer cell line BT-549_Myc9 with increased expression of MYC gene was established by CRISPR/SAM activation. BT-549_Myc9 cells showed an increased proliferation rate in vitro. MYC gene overexpression led to an increase in the CD44 + CD24 stem cell fraction and an increase in the number and diameter of mammospheres formed by BT-549_Myc9 cells. This effect was enhanced by IL6 stimulation. We hypothesized that MYC gene activation increases cancer cell plasticity and facilitates cell dedifferentiation, namely, facilitates the transition of a differentiated cancer cell into a stem cell