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Title: Определение чувствительности спорообразующих бактерий к антибиотику пиоцианину
Other Titles: Determination antibiotic sensitivity of spore-forming bacteria to pyocyanin
Authors: Хан, Е. А.
metadata.dc.contributor.advisor: Чубик, Марианна Валериановна
Keywords: бактерии; антибиотики; пиоцианин; микроорганизмы; инфекционные заболевания
Issue Date: 2019
Publisher: Изд-во ТПУ
Citation: Хан Е. А. Определение чувствительности спорообразующих бактерий к антибиотику пиоцианину / Е. А. Хан ; науч. рук. М. В. Чубик // Перспективы развития фундаментальных наук : сборник научных трудов XVI Международной конференции студентов, аспирантов и молодых ученых, г. Томск, 23-26 апреля 2019 г. : в 7 т. — Томск : Изд-во ТПУ, 2019. — Т. 4 : Биология и фундаментальная медицина. — [С. 134-136].
Abstract: Isolation of new antibiotics from substances that weren't previously used as antimicrobial drugs may be effective against resistant bacteria. Pseudomonas aeruginosa is a producer one of these substances. Pyocyanin is the blue-green pigment of the phenazine series and the secondary metabolite. In the present study, a five-day cultivation of P. aeruginosa, extraction and analysis of pyocyanine was carried out. Confirmation of the pyocyanin was done through UV-visible spectrophotometric analysis. In UV-Vis spectrophotometric analysis, a maximum absorption was observed at 690nm. Pyocyanin was used to determine the minimum inhibitory concentration against spore-forming bacteria Bacillus cereus and Bacillus subtilis. The serial dilution method showed that for Bacillus cereus the minimum inhibitory concentration of pyocyanin is 29 [mu]g/ml, and for Bacillus subtilis 17,8 [mu]g/ml. It was detected, that pyocyanin can suppress the growth of spore-forming bacteria.
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