Определение чувствительности спорообразующих бактерий к антибиотику пиоцианину

Abstract

Isolation of new antibiotics from substances that weren't previously used as antimicrobial drugs may be effective against resistant bacteria. Pseudomonas aeruginosa is a producer one of these substances. Pyocyanin is the blue-green pigment of the phenazine series and the secondary metabolite. In the present study, a five-day cultivation of P. aeruginosa, extraction and analysis of pyocyanine was carried out. Confirmation of the pyocyanin was done through UV-visible spectrophotometric analysis. In UV-Vis spectrophotometric analysis, a maximum absorption was observed at 690nm. Pyocyanin was used to determine the minimum inhibitory concentration against spore-forming bacteria Bacillus cereus and Bacillus subtilis. The serial dilution method showed that for Bacillus cereus the minimum inhibitory concentration of pyocyanin is 29 [mu]g/ml, and for Bacillus subtilis 17,8 [mu]g/ml. It was detected, that pyocyanin can suppress the growth of spore-forming bacteria.